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. Author manuscript; available in PMC: 2018 Sep 10.
Published in final edited form as: Cancer Lett. 2017 Jun 23;403:216–223. doi: 10.1016/j.canlet.2017.05.032

Figure 2. Modified technique for CSV marker staining in captured CTCs from osteosarcoma patient and normal PBMCs that have been cryopreserved and thawed.

Figure 2

(A–B) Immunofluorescent staining for 84-1 and CD45 antibody in both captured CTCs and normal immune cells after fixation. (C–D) Immunofluorescent staining for 84-1 and CD45 in both captured CTCs and normal immune cells before fixation (non-permeabilized cells). CSV (84-1, green), CD45 (red) and nuclear stain (blue). Scale indicates 10 μm.