Fig. 5.

- Validation of quantitative mass spectrometry findings via Western blotting. A, Western blotting analysis of HIV and host proteins in WT and ΔVPR Jurkat tetHIV inducible cell lines. B, Validation of tetHIV model using a SupT1 infection model. Similar protein expression patterns are observed, but there is a lag time from the infection process which required a later time point (48 h). C, Average mass spectrometry derived values for blots in A and B. Vpr was not detected in our proteomics experiments or the CEM-T4 experiments performed by Greenwood et al. Values are shown relative to the zero-time point, which was normalized to 1.