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. 2017 Jun 13;16(8):1462–1474. doi: 10.1074/mcp.M117.067488

Fig. 3.

Fig. 3.

Effect of selected novel GR-interacting proteins on GR target gene expression. A, Immunoblots from A549 and GR-expressing HEK293 cells after siRNA silencing of BCOR, IRF2BP2, KDM1A, RCOR1 or RAI1. Cells were transfected with specific siRNAs for 72 h and exposed for 6 h to 100 nm dexamethasone (D) or vehicle (V) before harvesting. Arrows depict specific bands for KDM1A and RCOR1 in A549 cells. B, Effect of the coregulator silencing on GR target gene expression. Gene expression levels were measured from vehicle- (white) or dexamethasone-treated (red, black) samples by RT-qPCR after silencing coregulator expression. Columns represent the mean ± S.D. of at least three biological replicates. Values are fold changes compared with siNON vehicle treatment in each cell line. Significances are for the difference between siNON and respective siRNA in the corresponding treatment in each cell line. ***, p < 0.001; **, p < 0.01; *, p < 0.05; ANOVA and Bonferroni.