Table I. List of RPE cell proteins with high affinity to galectin-1.

15 identified Gal-1 interactors in order of their enrichment factors in the β-Lactose eluates of galectin pulldowns compared with unspecific controls. For calculation the mean of all technical replicates within one experiment was used. Significance was determined by Student's t test. Proteins with p values of p < 0.05, enrichment of ≥2-fold in galectin pulldown eluates (FC ≥ 2), and additional identification in two or more independent pulldown experiments (n ≥ 2) were regarded as significant. The ratios represent the maximum fold changes of all experiments and the corresponding p values. TMD, transmembrane domain; SP, signal peptide; FC, fold change.

1st accession no.	Gene name	Ratio Gal-1/negative control	p value	TMD	SP	Protein name
ENSP00000333298	LAMP1	1065.8	0.006	1	Y	Lysosome-associated membrane protein 1
ENSP00000307513	MRC2	136.4	0.049	1	0	Mannose receptor, C-type 2
ENSP00000007722	ITGA3	104.6	0.021	1	Y	Integrin, α3
ENSP00000261023	ITGAV	59.2	0.016	1	Y	Integrin, αV
ENSP00000303351	ITGB1	53.9	0.014	1	Y	Integrin, β1
ENSP00000233714	LANCL1	52.2	0.001	0	0	LanC l antibiotic synthetase component C-like 1 (bacterial)
ENSP00000314508	GBA	34.8	0.000	0	Y	Glucosidase, β, acid
ENSP00000331544	FBLN1	34.1	0.018	0	Y	Fibulin 1
ENSP00000243077	LRP1	31.1	0.019	1	Y	Low-density lipoprotein receptor-related protein 1
ENSP00000258341	LAMC1	21.3	0.002	0	Y	Laminin, γ1 (formerly LAMB2)
ENSP00000262776	LGALS3BP	19.0	0.043	0	Y	Lectin, galactoside-binding, soluble, three binding proteins
ENSP00000228506	MLEC	18.1	0.037	1	Y	Malectin
ENSP00000265304	SSBP1	6.1	0.045	0	0	Single-stranded DNA- binding protein 1, mitochondrial
ENSP00000200639	LAMP2	5.9	0.001	1	Y	Lysosome-associated membrane protein 2
ENSP00000258733	GPNMB	5.3	0.049	1	Y	Glycoprotein (transmembrane) nmb