Table II. List of RPE cell proteins with high affinity to galectin-3.
131 identified Gal-3 interactors in order of their enrichment factors in the lactose eluates of galectin pulldowns compared with unspecific controls. For calculation, the mean of all technical replicates within one experiment was used. Significance was determined by Student's t test. Proteins with p values of p < 0.05, enrichment of ≥2-fold in galectin pulldown eluates (FC ≥ 2), and additional identification in two or more independent pulldown experiments (n ≥) were regarded as significant. The ratios represent the maximum fold changes of all experiments and the corresponding p values. TMD, transmembrane domain; SP, signal peptide; FC, fold change.
| 1st accession no. | Gene name | Ratio Gal3/neg. control | p value | TMD | SP | Protein name |
|---|---|---|---|---|---|---|
| ENSP00000273784 | AHSG | Infinity | 0.016 | 0 | Y | α2-HS-glycoprotein |
| ENSP00000222374 | CADM4 | Infinity | 0.047 | 1 | Y | Cell adhesion molecule 4 |
| ENSP00000312435 | DAG1 | Infinity | 0.004 | 1 | Y | Dystroglycan 1 (dystrophin-associated glycoprotein 1) |
| ENSP00000321573 | DCBLD2 | Infinity | 0.007 | 3 | Y | Discoidin, CUB, and LCCL domain containing 2 |
| ENSP00000334145 | F3 | Infinity | 0.003 | 1 | 0 | Coagulation factor III (thromboplastin, tissue factor) |
| ENSP00000314508 | GBA | Infinity | 0.014 | 0 | Y | Glucosidase, β, acid |
| ENSP00000282588 | ITGA1 | Infinity | 0.002 | 1 | Y | Integrin, α1 |
| ENSP00000264106 | ITGA6 | Infinity | 0.015 | 1 | Y | Integrin, α6 |
| ENSP00000266041 | ITIH4 | Infinity | 0.014 | 0 | Y | Inter-α-trypsin inhibitor heavy chain family, member 4 |
| ENSP00000258341 | LAMC1 | Infinity | 0.016 | 0 | Y | Laminin, γ1 (formerly LAMB2) |
| ENSP00000231004 | LOX | Infinity | 0.046 | 0 | Y | Lysyl oxidase |
| ENSP00000374135 | LRP1B | Infinity | 0.029 | 1 | Y | Low density lipoprotein receptor-related protein 1B |
| ENSP00000199940 | MAP2 | Infinity | 0.035 | 0 | 0 | Microtubule-associated protein 2 |
| ENSP00000217939 | MXRA5 | Infinity | 0.012 | 0 | Y | Matrix-remodeling associated 5 |
| ENSP00000294785 | NCSTN | Infinity | 0.009 | 1 | Y | Nicastrin |
| ENSP00000324270 | OXTR | Infinity | 0.041 | 7 | 0 | Oxytocin receptor |
| ENSP00000319782 | PODXL | Infinity | 0.020 | 1 | Y | Podocalyxin-like |
| ENSP00000356572 | QSOX1 | Infinity | 0.007 | 0 | Y | Quiescin Q6 sulfhydryl oxidase 1 |
| ENSP00000266771 | SLC15A4 | Infinity | 0.014 | 13 | 0 | Solute carrier family 15 (oligopeptide transporter), member 4 |
| ENSP00000444408 | SLC1A5 | Infinity | 0.017 | 9 | 0 | Solute carrier family 1 (neutral amino acid transporter), member 5 |
| ENSP00000004531 | SLC7A2 | Infinity | 0.042 | 14 | 0 | Solute carrier family 7 (cationic amino acid transporter, y+ system), member 2 |
| ENSP00000335300 | TPCN1 | Infinity | 0.002 | 12 | 0 | Two-pore segment channel 1 |
| ENSP00000351190 | ITIH2 | 527,961.6 | 0.006 | 0 | Y | Inter-α-trypsin inhibitor heavy chain 2 |
| ENSP00000418725 | ITGB1 | 20,223.9 | 0.011 | 0 | Y | Integrin, β1 (fibronectin receptor, β-polypeptide, antigen CD29 includes MDF2, MSK12) |
| ENSP00000269141 | CDH2 | 4103.6 | 0.003 | 1 | Y | Cadherin 2, type 1, N-cadherin (neuronal) |
| ENSP00000257857 | CD63 | 3186.1 | 0.032 | 4 | 0 | CD63 molecule |
| ENSP00000329797 | CADM1 | 2304.6 | 0.003 | 1 | Y | Cell adhesion molecule 1 |
| ENSP00000333298 | LAMP1 | 1591.0 | 0.003 | 1 | Y | Lysosome-associated membrane protein 1 |
| ENSP00000231368 | LNPEP | 1439.2 | 0.031 | 1 | 0 | Leucyl/cystinyl aminopeptidase |
| ENSP00000257879 | ITGA7 | 1433.6 | 0.000 | 1 | 0 | Integrin, α7 |
| ENSP00000256689 | SLC38A2 | 910.1 | 0.015 | 11 | 0 | Solute carrier family 38, member 2 |
| ENSP00000268613 | CDH13 | 900.6 | 0.008 | 0 | 0 | Cadherin 13 |
| ENSP00000368752 | PRNP | 737.3 | 0.024 | 2 | Y | Prion protein |
| ENSP00000263398 | CD44 | 581.2 | 0.017 | 1 | Y | CD44 molecule (Indian blood group) |
| ENSP00000382340 | ABCC1 | 521.0 | 0.002 | 10 | 0 | ATP-binding cassette, sub-family C (CFTR/MRP), member 1 |
| ENSP00000293379 | ITGA5 | 477.1 | 0.002 | 1 | Y | Integrin, α5 (fibronectin receptor, α-polypeptide) |
| ENSP00000230418 | PTK7 | 231.3 | 0.005 | 1 | Y | Protein-tyrosine kinase 7 |
| ENSP00000261978 | LTBP2 | 219.4 | 0.002 | 0 | Y | Latent transforming growth factor β-binding protein 2 |
| ENSP00000280527 | CRIM1 | 218.6 | 0.003 | 1 | Y | Cysteine-rich transmembrane BMP regulator 1 (chordin-like) |
| ENSP00000308727 | SUSD5 | 218.1 | 0.001 | 1 | Y | Sushi domain containing 5 |
| ENSP00000336888 | SLC44A2 | 208.8 | 0.006 | 10 | 0 | Solute carrier family 44 (choline transporter), member 2 |
| ENSP00000258733 | GPNMB | 202.7 | 0.007 | 1 | Y | Glycoprotein (transmembrane) nmb |
| ENSP00000256997 | ACP2 | 198.2 | 0.026 | 1 | Y | Acid phosphatase 2, lysosomal |
| ENSP00000357190 | PTPRK | 197.0 | 0.040 | 1 | Y | Protein-tyrosine phosphatase, receptor type, K |
| ENSP00000356787 | ATP1B1 | 188.5 | 0.044 | 1 | 0 | ATPase, Na+/K+-transporting, β1 polypeptide |
| ENSP00000310206 | SEZ6L2 | 184.0 | 0.041 | 1 | Y | Seizure-related 6 homolog (mouse)-like 2 |
| ENSP00000306864 | VASN | 180.9 | 0.024 | 1 | Y | Vasorin |
| ENSP00000348307 | SIRPA | 146.7 | 0.044 | 1 | Y | Signal-regulatory protein α |
| ENSP00000421922 | LRPAP1 | 141.9 | 0.001 | 0 | Y | Low density lipoprotein receptor-related protein-associated protein 1 |
| ENSP00000320084 | CD276 | 121.5 | 0.010 | 1 | Y | CD276 molecule |
| ENSP00000305988 | ALCAM | 121.5 | 0.007 | 1 | Y | Activated leukocyte cell adhesion molecule |
| ENSG00000125730 | C3 | 119.1 | 0.042 | 0 | Y | Complement component 3 |
| ENSP00000290401 | NPTN | 115.6 | 0.002 | 1 | Y | Neuroplastin |
| ENSP00000378392 | PSAP | 111.3 | 0.002 | 0 | Y | Prosaposin |
| ENSP00000264036 | MCAM | 109.0 | 0.022 | 1 | Y | Melanoma cell adhesion molecule |
| ENSP00000311502 | HEG1 | 101.8 | 0.003 | 0 | 0 | Heart development protein with EGF-like domains 1 |
| ENSP00000331544 | FBLN1 | 100.3 | 0.015 | 0 | Y | Fibulin 1 |
| ENSP00000311402 | SLC4A2 | 96.9 | 0.046 | 13 | 0 | Solute carrier family 4 (anion exchanger), member 2 |
| ENSP00000228506 | MLEC | 96.6 | 0.000 | 1 | Y | Malectin |
| ENSP00000053867 | GRN | 85.3 | 0.013 | 0 | Y | Granulin |
| ENSP00000296181 | ITGB5 | 84.8 | 0.001 | 1 | Y | Integrin, β5 |
| ENSP00000265077 | VCAN | 83.4 | 0.003 | 0 | Y | Versican |
| ENSP00000295633 | FSTL1 | 77.8 | 0.008 | 0 | Y | Follistatin-like 1 |
| ENSP00000413922 | ITIH3 | 76.7 | 0.024 | 0 | Y | Inter-α-trypsin inhibitor heavy chain 3 |
| ENSP00000352288 | PLXNB2 | 75.0 | 0.005 | 1 | Y | Plexin B2 |
| ENSP00000318557 | SLC12A4 | 74.5 | 0.013 | 14 | 0 | Solute carrier family 12 (potassium/chloride transporter), member 4 |
| ENSP00000324101 | CD151 | 72.1 | 0.028 | 4 | 0 | CD151 molecule (Raph blood group) |
| ENSP00000266718 | LUM | 65.3 | 0.001 | 0 | Y | Lumican |
| ENSP00000273258 | ARL6IP5 | 60.8 | 0.049 | 4 | 0 | ADP-ribosylation-like factor 6 interacting protein 5 |
| ENSP00000269228 | NPC1 | 60.7 | 0.009 | 13 | Y | Niemann-Pick disease, type C1 |
| ENSP00000347596 | EFEMP1 | 57.1 | 0.004 | 0 | Y | EGF-containing fibulin-like extracellular matrix protein 1 |
| ENSP00000333697 | TMEM179B | 52.2 | 0.007 | 3 | Y | Transmembrane protein 179B |
| ENSP00000312506 | CSPG4 | 44.1 | 0.003 | 1 | Y | Chondroitin sulfate proteoglycan 4 |
| ENSP00000188790 | FAP | 43.6 | 0.022 | 0 | Y | Fibroblast activation protein, α |
| ENSP00000323534 | FN1 | 43.3 | 0.002 | 0 | Y | Fibronectin 1 |
| ENSP00000262776 | LGALS3BP | 41.9 | 0.004 | 0 | Y | Lectin, galactoside-binding, soluble, 3 binding protein |
| ENSP00000243077 | LRP1 | 41.5 | 0.001 | 1 | Y | Low density lipoprotein receptor-related protein 1 |
| ENSP00000315130 | CLU | 39.7 | 0.002 | 0 | Y | Clusterin |
| ENSP00000206423 | CCDC80 | 38.8 | 0.001 | 0 | Y | Coiled-coil domain containing 80 |
| ENSP00000318646 | RPS15A | 37.8 | 0.003 | 0 | 0 | Ribosomal protein S15a |
| ENSP00000200639 | LAMP2 | 37.6 | 0.004 | 1 | Y | Lysosome-associated membrane protein 2 |
| ENSP00000307513 | MRC2 | 37.5 | 0.002 | 1 | 0 | Mannose receptor, C type 2 |
| ENSP00000261023 | ITGAV | 32.1 | 0.015 | 1 | Y | Integrin, αV |
| ENSP00000341730 | RPL10 | 31.6 | 0.003 | 0 | 0 | Ribosomal protein L10 |
| ENSP00000371626 | TRA2B | 31.4 | 0.006 | 0 | 0 | Transformer 2 βhomolog (Drosophila) |
| ENSP00000323929 | A2M | 30.9 | 0.002 | 0 | Y | α2-Macroglobulin |
| ENSP00000333769 | BSG | 26.8 | 0.000 | 1 | Y | Basigin (Ok blood group) |
| ENSP00000007722 | ITGA3 | 26.7 | 0.001 | 1 | Y | Integrin, α3 (antigen CD49C, α3 subunit of VLA-3 receptor) |
| ENSP00000349437 | IGF2R | 26.5 | 0.003 | 1 | Y | Insulin-like growth factor 2 receptor |
| ENSP00000084795 | RPL18 | 25.6 | 0.000 | 0 | 0 | Ribosomal protein L18 |
| ENSP00000222399 | LAMB1 | 24.9 | 0.000 | 0 | Y | Laminin, β1 |
| ENSP00000226359 | AFP | 22.1 | 0.013 | 0 | Y | α-Fetoprotein |
| ENSP00000252804 | PXDN | 21.3 | 0.006 | 0 | Y | Peroxidasin |
| ENSP00000275730 | SLC12A9 | 20.8 | 0.003 | 13 | 0 | Solute carrier family 12, member 9 |
| ENSP00000359602 | LMBRD1 | 19.6 | 0.013 | 7 | 0 | LMBR1 domain containing 1 |
| ENSP00000366460 | PLXDC2 | 19.2 | 0.007 | 1 | Y | Plexin domain containing 2 |
| ENSP00000286371 | ATP1B3 | 18.8 | 0.035 | 1 | 0 | ATPase, Na+/K+-transporting, β3 polypeptide |
| ENSP00000264896 | SCARB2 | 15.7 | 0.026 | 1 | Y | Scavenger receptor class B, member 2 |
| ENSP00000340815 | SLC3A2 | 15.6 | 0.012 | 1 | 0 | Solute carrier family 3 (amino acid transporter heavy chain), member 2 |
| ENSP00000327290 | ITGA11 | 15.2 | 0.016 | 1 | Y | Integrin, α11 |
| ENSP00000260356 | THBS1 | 15.2 | 0.002 | 0 | Y | Thrombospondin 1 |
| ENSP00000341861 | SERPING1 | 13.7 | 0.041 | 0 | Y | Serpin peptidase inhibitor, clade G (C1 inhibitor), member 1 |
| ENSP00000376899 | PTGFRN | 13.3 | 0.016 | 1 | Y | Prostaglandin F2 receptor inhibitor |
| ENSP00000377047 | PTPRZ1 | 13.1 | 0.048 | 1 | Y | Protein-tyrosine phosphatase, receptor-type, Z polypeptide 1 |
| ENSP00000296585 | ITGA2 | 12.1 | 0.003 | 1 | Y | Integrin, α2 (CD49B, α2 subunit of VLA-2 receptor) |
| ENSP00000261799 | PDGFRB | 11.7 | 0.012 | 1 | Y | Platelet-derived growth factor receptor, β-polypeptide |
| ENSP00000355330 | TGM2 | 11.4 | 0.001 | 0 | 0 | Transglutaminase 2 |
| ENSP00000222247 | RPL18A | 10.2 | 0.001 | 0 | 0 | Ribosomal protein L18a |
| ENSP00000344456 | CTNNB1 | 9.1 | 0.000 | 0 | 0 | Catenin (cadherin-associated protein), β1, 88 kDa |
| ENSP00000272317 | RPS27A | 8.8 | 0.006 | 0 | 0 | Ribosomal protein S27a |
| ENSP00000264832 | ICAM1 | 6.7 | 0.000 | 1 | Y | Intercellular adhesion molecule 1 |
| ENSP00000296674 | RPS23 | 6.4 | 0.016 | 0 | 0 | Ribosomal protein S23 |
| ENSP00000346015 | RPL27A | 5.9 | 0.000 | 0 | 0 | Ribosomal protein L27a |
| ENSP00000369743 | RPS6 | 4.8 | 0.001 | 0 | 0 | Ribosomal protein S6 |
| ENSP00000253788 | RPL27 | 4.8 | 0.003 | 0 | 0 | Ribosomal protein L27 |
| ENSP00000416293 | SLC2A1 | 4.5 | 0.010 | 12 | 0 | Solute carrier family 2 (facilitated glucose transporter), member 1 |
| ENSP00000311430 | RPL4 | 4.3 | 0.027 | 0 | 0 | Ribosomal protein L4 |
| ENSP00000277865 | GLUD1 | 4.2 | 0.036 | 0 | Y | Glutamate dehydrogenase 1 |
| ENSP00000345689 | RAB5C | 4.2 | 0.026 | 0 | 0 | RAB5C, member RAS oncogene family |
| ENSP00000363018 | RPL10A | 3.8 | 0.006 | 0 | 0 | Ribosomal protein L10a |
| ENSP00000348849 | RPS26 | 3.8 | 0.024 | 0 | 0 | Ribosomal protein S26 |
| ENSP00000225698 | C1QBP | 3.4 | 0.027 | 0 | 0 | Complement component 1, q subcomponent binding protein |
| ENSP00000346022 | RPL9 | 3.4 | 0.011 | 0 | 0 | Ribosomal protein L9 |
| ENSP00000295598 | ATP1A1 | 3.3 | 0.003 | 8 | 0 | ATPase, Na+/K+-transporting, α1 polypeptide |
| ENSP00000379888 | RPS8 | 3.2 | 0.026 | 0 | 0 | Ribosomal protein S8 |
| ENSP00000305920 | GLB1 | 3.1 | 0.047 | 0 | Y | Galactosidase, β1 |
| ENSP00000325136 | HADHB | 3.1 | 0.030 | 0 | Y | Hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), β subunit |
| ENSP00000346027 | RPL21 | 3.1 | 0.036 | 0 | 0 | Ribosomal protein L21 |
| ENSP00000366156 | SRM | 3.1 | 0.038 | 0 | 0 | Spermidine synthase |
| ENSP00000251453 | RPS16 | 2.5 | 0.040 | 0 | 0 | Ribosomal protein S16 |
| ENSP00000342070 | CTSB | 2.5 | 0.004 | 0 | Y | Cathepsin B |