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. Author manuscript; available in PMC: 2018 Jul 7.
Published in final edited form as: Environ Microbiol. 2017 Jul 7;19(7):2831–2842. doi: 10.1111/1462-2920.13808

Figure 1. Ribonuclease assay of VapCs found to degrade total RNA from M. sedula and M. prunae.

Figure 1

Total RNA harvested at mid-exponential phase from M. sedula and M. prunae treated with (A) VapC4 (Msed_0899), (B) VapC7 (Msed_1214) and (C) VapC8 (Msed_1245). Only three out of the 13 VapC toxins/PIN domain proteins were found to degrade total RNA and their activity could be quenched by the cognate VapB. Mass ratios of VapC:RNA of 1:20, 1:50, 1:50 and reactions times of 45 min, 30 min and 45 min were used for VapC4, VapC7 and VapC8, respectively. The VapC:VapB mass ratio was 1:2 for all VapCs. Key: ‘-VapC’ = No VapC added; ‘EDTA’ = 25 mM EDTA + VapC; ‘VapBC’ = VapB + VapC added; ‘VapC’ = VapC added, no EDTA, no VapB.