Skip to main content
. Author manuscript; available in PMC: 2018 Mar 16.
Published in final edited form as: Mol Cell. 2017 Mar 16;65(6):1122–1135.e5. doi: 10.1016/j.molcel.2017.02.008

Figure 4. IKKβ phosphorylation of S305 ERα is required for cytokine-dependent ERα activation.

Figure 4

(A) Western blot analysis for ERα-S305-P and ERα in MCF-7 cells treated with Veh, or IL1β in the absence or presence of inhibitors for PKA, PAK1and IKKα/β.

(B) Quantification of two independent western blot analysis as performed in figure 4A. Values are expressed as mean ± SEM.*p<0.05, (Student’s t-test) compared to IL1β sample.

(C) Western blot analysis for ERα-S305-P and total ERα in MCF-7 cells treated with siRNA for Ctl, IKKα, or IKKβ and treated with Veh or IL1β.

(D) Histogram of ERα ChIP-Seq signal in the presence of Veh, IL1β, IKK7, or a combination ofIL1β andIKK7.

(E) ChIP-PCR of ERα recruitment to the MYC genomic locus in the presence of Veh, IL1β, or a combination of IL1β and IKK7. Values are expressed as mean ± SEM. *p<0.05, (Student’s t-test) IL1β + IKK7 compared to IL1β sample.

(F) Quantitative real-time PCR data for MYC mRNA in MCF-7 cells treated with Veh, IL1β, or a combination of IL1β and IKK7. Values are expressed as mean ± SEM.*p<0.05, (Student’s t-test) IL1β +IKK7 compared to IL1β sample.

(G) Same as F, but cells were also treated with TOT or TNFα, as indicated.