REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-Myc	Santa Cruz	9E10; Cat#SC-40
Goat polyclonal anti-lamin B1	Santa Cruz	C20; Cat#SC-6216
Rabbit polyclonal anti-Gαt1	Santa Cruz	K20; Cat#SC-389
Goat polyclonal anti-rootletin	Santa Cruz	C20; Cat#SC-67824
Mouse monoclonal anti-EGFP	Santa Cruz	B2; Cat#SC-9996
Rabbit polyclonal anti-Cone arrestin (CAR)	Millipore	Cat#AB15282
Mouse monoclonal anti-Centrin	Millipore	Clone 20H5; Cat#04-1624
Mouse monoclonal anti-gamma tubulin	Sigma-Aldrich	Clone GTU-88; Cat#T6557
Mouse monoclonal anti-acetylated tubulin	Sigma-Aldrich	Clone 6-11B-1; Cat #T7451
Mouse polyclonal anti-Nesprin1	[23]	Nes1M5
Rabbit polyclonal anti-Nesprin1	[23]	Nes1HAA12
Rabbit polyclonal anti-Nesprin2	[49]	Nes2K2
Rabbit polyclonal anti-Sun1	[23]	Sun1RFA1
Rabbit polyclonal anti-Sun2	This paper	Sun2CCA13
Alexa594-conjugated donkey anti-Rabbit	Thermofisher	Cat#A21207
Alexa594-conjugated donkey anti-Goat	Thermofisher	Cat#A11058
Alexa594-conjugated donkey anti-mouse	Thermofisher	Cat#A21203
Alexa488-conjugated donkey anti-Rabbit	Thermofisher	Cat#A21206
Alexa488-conjugated donkey anti-Goat	Thermofisher	Cat#A11055
Alexa488-conjugated donkey anti-mouse	Thermofisher	Cat#A21202
Alexa680-conjugated donkey anti-mouse	Thermofisher	Cat#A10038
Rabbit Fab Alexa 594 Fluoronanogold particles	Nanoprobes	Cat#7304
Chemicals, Peptides, and Recombinant Proteins
Ketamine	Putney	Cat#26637041101
Xylazine	Santa Cruz Biotechnology	Cat#sc-253838
Atropin sulfate	Bausch&Lomb	Cat#AB05007
Gonak	Akorn	Cat#17478006412
DMEM	Sigma-Aldrich	Cat#D5796
Fetal Bovine serum	Atlanta Biologicals, Inc	N/A
100XPenicillin/Streptomycin	Thermofisher	Cat#15140122
Paraformaldehyde	Electron Microscopy Sciences	Cat#15710
Phosphate Buffer Saline	Thermofisher	Cat#14200-075
Lipofectamine SiRNA Max	Thermofisher	Cat#13778-030
OCT compound	Tissue-Tek	Cat#4583
Donkey serum	Sigma-Aldrich	Cat#D9663
Triton X-100	Sigma-Aldrich	Cat#T8284
4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI)	Sigma-Aldrich	Cat#32670
Fluorescent mounting medium	DAKO	Cat#S3023
Optimem	Thermofisher	Cat#11058-021
Lipofectamine 2000	Thermofisher	Cat#11668-027
Glutaraldehyde	Electron Microscopy Sciences	Cat#16216
Araldite resin	Electron Microscopy Sciences	Cat#13940
Low melt agarose	Sigma-Aldrich	Cat#A9414
Protease inhibitors	Roche	Cat#11836153001
Super Signal West Pico	Thermofisher	Cat#1856135
Protein A/G beads	Pierce	Cat#20423
Trizol	Thermofisher	Cat#15596020
Zirconium oxide beads	Next Advance	Cat#18064014
Superscript II	Thermofisher	Cat#
Taq HI-FI polymerase	Thermofisher	Cat#11304-011
Pronase E	Sigma-Aldrich	Cat# P5147
F12K nutrient mixture	Thermofisher	Cat# 21127030
DNase I	Sigma-Aldrich	Cat# DN25
DMEM/F12	Thermofisher	Cat# 11330-032
L-Glutamine	Thermofisher	Cat# 25030-149
Sodium bicarbonate	Thermofisher	Cat# 25080-094
Insulin	Sigma-Aldrich	Cat# I-6634
Epidermal growth factor	BD Biosciences	Cat# 354001
Apo-Transferrin	Sigma-Aldrich	Cat# T1147
Cholera toxin	Sigma-Aldrich	Cat# C8052
Bovine pituitary extract	Hammond Cell Tech	Cat# 1077
Retinoid acid	Sigma-Aldrich	Cat# R-2625
Transwell-Clear permeable filter supports	Corning	Cat# 3470
Fungizone	Thermofisher	Cat# 15290-018
Experimental Models: Cell Lines
NIH3t3	ATCC	ATCC#CRL-1658
Experimental Models: Organisms/Strains
Mouse: Rho-Cre	[18]	JAX#015850
Mouse: Rho−/−	[45]	N/A
Mouse: Actin-Cre	[46]	JAX#003376
Mouse:Chx10-Cre	[29]	JAX#005105
Mouse: Nesprin1f/f	[19]	N/A
MOUSE: CAG-LacZ/EGFP-KASH2	[50]	N/A
Oligonucleotides
Nesprin1 DsiRNAs, 27 mer duplexes, TrifectA RNAi kit	Integrated DNA Technologies	mm.Ri. Syne1.13
Nesprin1α 5′primer: 5′-TCCAGGAAGGACTGAGCCTT-3′	Integrated DNA Technologies	N/A
Nesprin1Long 5′ primer: 5′GAGAACAAAATCCAGCTTCAGGAAATGGGAGAACGGC-3′	Integrated DNA Technologies	N/A
Nesprin1 3′ primer: 5′-CTTGTGTGGGGATGTCTGCT-3′	Integrated DNA Technologies	N/A
Recombinant DNA
Myc-Rootletin	[30]	Addgene plasmid#41167
Root C1-2	This paper	N/A
Root C1-3	This paper	N/A
Root C1-4	This paper	N/A
EGFP-Nes1α	This paper	N/A
EGFP-Nes1αΔKASH	This paper	N/A
EGFP-KASH2	[27]	N/A
EGFP-KASH2ext	[27]	N/A
Software and Algorithms
NIS-Elements, Nikon	N/A	N/A
TopHat2 V.2.0.5	[54]	N/A
Bowtie V.0.12.8	[55]	N/A
Samtools V.0.1.18	[56]	N/A
Bedtools v.2.23.0	[58]	N/A
Integrative genomic viewer	[57]	N/A
Allen Mouse Brain Atlas	[31]	N/A
Origin 8.5	OriginLab	N/A