Table 3. Sequences of SCAR primers, PCR product size (bp).
SCAR | 5′-primer | Sequence (5′-3′) | 3′-primer | Sequence (5′-3′) | Size | Tm (°C) |
---|---|---|---|---|---|---|
BC10-1 | BC10-1L | GATCACAGGCAGTCAGAGAGG | BC10-1R | CAGGCCACTTGAGACTCTCTG | 590 | 60 |
BC31-2 | BC31-2L | ACAAACGGCCCCATTTCTTTG | BC31-2R | TTGGTGGTTGTGGTAGATGGG | 512 | 60 |
GAPDH | GAPDH5G | ACCCAGAAGACTGTGGATGG | GAPDH3G | TGACAAAGTGGTCGTTGAGG | 376 | 60 |
Abbreviations: SACR, sequence-characterized amplified region; bp, base pair.