Figure 3.

The amplified fragments of RT-PCR for top six overexpressed (A) including TOP2A, GPT2, GINS2, HJURP, TK1, and CDCA5 and underexpressed (B) including AGER, FHL1, CLDN18, ADAMTS8, ADH1B, and GPIHBP1) mRNAs. Weakly positive bands of GPT2, HJURP and ADAMTS8 were identified in cfRNA samples from lung cancer patient and healthy subject after separation in an agarose gel respectively. (C) GAPDH was amplified as the control PCR product. N represents cfRNA from healthy subject. LC represents cfRNA from lung cancer patient. NC represents no template control. A594 represent total RNA from A594 cells as positive control.