Table 4. Primers for candidate reference genes.

Gene	Primer sequence (5'→3')	Ampliconlength (bp)	Gene name	Function
TBP	F: CAG CAT CAC TGT TTC TTG GCG TR: AGA TAG GGA TTC CGG GAG TCA T	232	TATA-box binding protein	General transcription factor
HPRT1	F: CAG CCC TGG CGT CGT GAT TAG TR: CCA GCA GGT CAG CAA AGA AT	226	Hypoxanthine-guanine phosphoribosyltransferase	Metabolic salvage of purines
RPL13A	F: CCT GGA GGA GAA GAG GAA AGA GAR: TTG AGG ACC TCT GTG TAT TTG TCA A	126	60s ribosomal protein L13a	Component of 60Sribosomal unit
GAPDH	F: TCG CCA GCC GAG CCA CAT CR: CGT TCT CAG CCT TGA CGG TGC	222	Glyceraldehyde-3-phosphatedehydrogenase	Glycolysis enzyme
CYC1	F: GAG GTG GAG GTT CAA GAC GGR: TAG CTC GCA CGA TGT AGC TG	160	Cytochrome C-1	Mitochondrial electron transport

F, forward; R, reverse