Skip to main content
. 2017 Apr 24;8(27):44171–44185. doi: 10.18632/oncotarget.17396

Figure 2. Evaluation of siRNA effectiveness in CRC cell lines (THC8307 and THC8307/Oxa).

Figure 2

(A-B) WB technique showed that PKM2 expression was significantly inhibited after PKM2-siRNA (siPKM2) in the THC8307 cell line. (C-D) WB technique showed that GLS1 expression was significantly inhibited after GLS1-siRNA (siGLS1) in the THC8307 cell line. The expression of PKM2/GLS1 was remarkably decreased in the siRNA group, compared with that in the NCsiRNA group (**p < 0.01). (E-F) qRT-PCR technique confirmed PKM2/GLS1 mRNA expression was significantly inhibited after PKM2-siRNA (siPKM2)/GLS1-siRNA (siGLS1) in the THC8307/Oxa cell line, which did not affect GLS1/PKM2 mRNA expression (**p < 0.01). The combination of PKM2-siRNA with GLS1-siRNA achieved the optimal siRNA efficiency in the downregulation of PKM2/GLS1 (**p < 0.01). (G) WB technique confirmed the siRNA efficiency in PKM2/GLS1 downregulation in the THC8307/Oxa cell line.