Figure 1. Confirmation of proteins variation by target analysis.

SRM-LC-MS/MS analysis confirmed previous variations identified by iTRAQ for three representative proteins showing significant alteration in response to dnA (early response): MPO (A), OLFM4 (B) and AT3 (C). Non-parametric Kruskal-Wallis test with Dunn's multiple comparisons post-test and 95% confidence level was performed between each pathological condition (N, dnA, MHA) and control (C). (Panels D to F) show receiver operating curves (ROC) for these proteins, respectively; t-test values were 0.029 (MPO), 0.0089 (AT3) and 0.0097 (OLFM4).