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. 2017 May 15;8(27):44312–44325. doi: 10.18632/oncotarget.17871

Figure 4. The subpopulation of EpCAM-positive ovarian cancer cells prevents platinum anticancer drug-induced apoptosis.

Figure 4

To compare the anti-apoptotic ability of EpCAM-positive and EpCAM-negative cancer cells, we sorted EpCAM-positive and EpCAM-negative cells from the A2780 ovarian cancer cell line (A) and SKOV3 cell line (B). FACS-sorted EpCAM-positive and -negative cells were treated with18 μM cisplatin for 24 h, and the expression of apoptosis-associated proteins, including bcl-2, bax, caspase-3, and poly (ADP-ribose) polymerase (PARP), were examined by western blot analysis. (C) Representative immunohistochemical EpCAM and Bcl-2 staining in serial sections of ovarian cancer specimens. Bcl-2 was mainly detected EpCAM-positive cancer cells (arrows) (Scale bar: 100 μm). (D) Immunoblot analysis of EpCAM, Bcl-2, and Bax in A2780 cells transfected EpCAM or control siRNAs. (E) Immunoblot analysis of EpCAM, Bcl-2, and Bax in A2780 cells transduced overexpressing EpCAM (pCMV6-EpCAM expression vector) and their respective controls (pCMV6 empty vector). (F) A2780 cells transfected with EpCAM or control siRNAs were subjected to immunoblot analysis with indicated antibodies.