Table 3. Primers used in this study.
Genes | No. | Nucleotide sequence of primers (5′–3′) | Size (bp) |
---|---|---|---|
β-Actin | NM_031144.3 | F: CTGTGTGGATTGGTGGCTCT R: CAGCTCAGTAACAGTCCGCC |
135 |
IL-1β | NM_031512.2 | F: GACTTCACCATGGAACCCGT R: CAGGGAGGGAAACACACGTT |
200 |
IL-4 | NM_201270.1 | F: CGTGATGTACCTCCGTGCTT R: ATTCACGGTGCAGCTTCTCA |
108 |
IL-6 | NM_012589.2 | F: AGCGATGATGCACTGTCAGAA R: GCATTGGAAGTTGGGGTAGGA |
281 |
IL-10 | NM_012854.2 | F: CCTGGTAGAAGTGATGCCCC R: GATGCCGGGTGGTTCAATTT |
281 |
IL-17 | NM_001106897.1 | F: TCCTCTATTGTCCGCCATGC R: ATTTGTATCCCCTCTGCGCC |
194 |
TNF-α | XM_008772775.2 | F: AAGCTGTCTTCAGGCCAACA R: CCCGTAGGGCGATTACAGTC |
233 |
NQO1 | NM_017000.3 | F: GGAGACTGTCTGGGAGGAGT R: GCTTTGATCTGGTTGTCGGC |
180 |
NQO2 | NM_001004214.1 | F: TCCAGGAGGCAGAGACTGTT R: AGAGGGCACCAGTAACATCG |
276 |
CAT | NM_012520.2 | F: TTTTCACCGACGAGATGGCA R: CCCACAAGGTCCCAGTTACC |
274 |
GPX1 | NM_030826.4 | F: AGTGCGAGGTGAATGGTGAG R: GGAATGCCTTAGGGGTTGCT |
280 |
GPX2 | NM_183403.2 | F: GCATGGCTTACATCGCCAAG R: GGAAGCCGAGAACCACTAGG |
201 |
SOD1 | NM_017050.1 | F: AGGGCGTCATTCACTTCGAG R: CCTCTCTTCATCCGCTGGAC |
194 |
SOD2 | NM_017051.2 | F: ACGCGACCTACGTGAACAAT R: GCCTCCAGCAACTCTCCTTT |
196 |
F: forward; R: reverse; IL: interleukin; TNF-α: tumor necrosis factor; NQO: NAD(P)H quinone dehydrogenase; CAT: catalase; GPX: glutathione peroxidase; SOD: superoxide dismutase