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. 2017 May 18;8(27):44447–44464. doi: 10.18632/oncotarget.17968

Figure 2. VDRM2-dependent VDR activity is sustained in VCaP cells.

Figure 2

(A, B) VCaP cells were treated with vehicle (EtOH), 30 nM 1,25D(OH)2D3 (1,25D) or 300 nM VDRM2 for 96 or 24 hours, harvested, and RNA was purified. VDR target genes CYP24A1 and TRPV6 were measured using RT-qPCR and normalized to 18S. (C, D) VCaP cells were treated with vehicle (EtOH), 100 nM or 300 nM 1,25D(OH)2D3 for 96 or 24 hours, harvested, and RNA purified. VDR target genes CYP24A1 and TRPV6 were measured using RT-qPCR and normalized to 18S. **p < 0.01, ***p < 0.001, relative to respective 24 hour time point. n = 3, representative graph, mean ± SEM.