Figure 5. IL-17 reduces HCC cell apoptosis by activating CMA.

(A) HepG2 and SMMC-7721 cells were co-cultured with M2-TAMs for 24 h and then treated with 20 μg/mL oxaliplatin for various times. LAMP-2A and HSC70 levels were detected by Western blotting. (B) HepG2 and SMMC-7721 cells were cultured with or without M2-TAMs for 24 h and then treated with 20 μg/mL oxaliplatin for another 24 h. LAMP-2A and HSC70 levels were detected by Western blotting. (C) sh-IL-17R HepG2 cells were cultured with or without M2-TAMs for 24 h and then treated with 20 μg/mL oxaliplatin for another 24 h. LAMP-2A and HSC70 levels were detected by Western blotting. (D and E) HepG2 cells were infected with lentiviruses expressing a specific shRNA (sh-LAMP-2A). The transfection efficiency was detected by fluorescence microscopy (200×) (D) and the knockdown efficiency was detected by Western blotting (E). (F) sh-LAMP-2A HepG2 cells were cultured with or without M2-TAMs for 24 h and then treated with 20 μg/mL oxaliplatin for another 24 h. BAX, BCL-2, caspase-3 and cleaved caspase-3 were detected by Western blotting.