Figure 5. Effect of HIF-1α gene silencing on the migration and in vivo metastatic potential.

(A-B), Baseline motility of HT168-M1 and HT29 tumor cell lines measured under normoxic (24 h) and hypoxic (1% O₂) conditions for 72 h using time-lapse videomicroscopy. Curves represent the mean ± SD of migrated distance during the test period. (C), HIF-1α silenced HT168-M1 cells were injected intrasplenically and liver colonies formed were counted at day 34 of the treatment. Hypoxia means CoCl₂ treatment. Data represent mean ± SEM, *p<0.05.