Figure 3.

Analysis of plasmid-encoded rRNA containing mutations that shifted the relative positions of the bulged nucleotides. (A) Schematic representation of the mutations and the name by which they are referred to in the text. Schematics coded by color and text as indicated in Figure 2. Maturation of rRNA scored as (+) normal processing with accumulation of mature 25S; (+/–) no mature 25S but significant accumulation of stable precursors; (–) low or no detectable levels of mature 25S. Below, northern blot analysis of mutations illustrated in (A). Gel was manipulated as indicated in Figure 2. (B) Longer exposure of (C) presented to visualize the lower amounts of precursors and mature 25S present in some of the mutants; (D) probe for PGK1. (E) Separate experiment where the PGK1 levels for +2P are much more equivalent to that in WT. Processing is less efficient, but mature 25S clearly accumulates.