Figure 5.

Analysis of plasmid-encoded rRNA containing mutations that altered the mature termini. (A) Schematic representation of the mutations and the name by which they are referred to in the text. Schematics coded by color and text as indicated in Figure 2. Maturation of rRNA scored as (+) normal processing with accumulation of mature 25S; and (–) low or no detectable levels of mature 25S. Below, northern blot analysis of mutations illustrated in (A). Gel was manipulated as indicated in Figure 2. (C) Longer exposure of (B) presented to visualize the lower amounts of precursors and mature 25S present in some of the mutants; (D) probe for PGK1.