Figure 1. A panel of breast cancer cell lines expresses GRM1.

Estrogen receptor (ER) positive (MCF-7, T-47D, BT-474) and ER negative (MDA-MB-231, Hs578T, BT-549) breast cancer cell lines were tested for GRM1 expression by Western blot. C8161 (GRM1+) and UACC930 (GRM1 C-terminal truncation) melanoma cells were included as a positive and negative control, respectively, for GRM1 expression at the predicted molecular weight (MW) of 132 kilodaltons (kDa). β-actin served as a loading control.