Figure 8. Riluzole-mediated G2/M arrest is independent of induction of oxidative stress.

Breast cancer cell lines treated with 50 μM riluzole or 50 μM BAY 36-7620 for 24 h were evaluated for levels of reactive oxygen species (ROS) (A) and total intracellular glutathione (GSH) (B). Relative fold change was compared to DMSO control. *P < 0.05 compared to DMSO control. **P < 0.005 compared to DMSO control. Cell cycle distribution (represented as the percentage of cells in G2/M) was detected by flow cytometry in cells pretreated with 5mM N-acetyl-cysteine (NAC) followed by the addition of 25 μM H₂O₂ (C) or 50 μM riluzole (D). *P < 0.05 compared to control. **P < 0.005 compared to control. ‡ P < 0.005 for NAC+H₂O₂ compared to H₂O₂ treatment alone. Not significant (n.s.). Data are represented as mean +/− SD.