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The effect of the length and the structure of TAR RNA on the solubility of Tat-EGFP. (A) The predicted secondary structure of the 5’-end of the HIV-1 transcript including the 5-proximal TAR sequence84 and the relative solubility of Tat protein upon co-expression with TAR RNAs of varying size. (B) SDS-PAGE gel shows the wild type Tat protein co-expressed with TAR RNAs of various size: 57, 80, or 104 nt in length.
