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. 2017 Aug 7;12(8):e0181802. doi: 10.1371/journal.pone.0181802

Fig 3. Comparison of TUNEL results obtained with the two kits.

Fig 3

(A) Total percentage of TUNEL-positive spermatozoa; (B) Percentage of PI-dimmer population positive for TUNEL; (C) Percentage of PI-brighter population positive for TUNEL. Direct labeling: grey columns; Indirect labeling: white columns. For each semen sample the percentage of immotile spermatozoa calculated in the conventional semen analysis and the percentage of PI-dimmer population obtained after PI-staining during TUNEL assay are indicated in the X-axis, in the first sample, 38/20 corresponds to a sample with 38% of immotile spermatozoa and 20% of PI dimmer spermatozoa. The non-normozoospermic men (2010 WHO reference values) are indicated with an asterisk (S1 Table). CI-confidence interval, p-Wilcoxon Signed Rank p.