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. 2017 Aug 7;12(8):e0182641. doi: 10.1371/journal.pone.0182641

Fig 2. The coiled-coil interaction of CC1 plays an essential role in N1-mediated crosslinking of dynamic MTs.

Fig 2

(A) Schematic representation of the N-terminal mutants used in the following experiments and the summary of results. Asterisks indicate previously reported results [21]. ND indicates not determined. (B) Immunostaining of V5 and α-tubulin in HeLa-K cells expressing V5-N1 wild-type, 5LP, or 5LA. The bottom panel is an enlarged view of the rectangle region of V5-N1 5LA-expressing cells. Scale bars, 10 μm. (C) Immunostaining of GFP, α-tubulin, and acetylated tubulin in HeLa-K cells expressing GFP, GFP-hCMTBD, and GFP-N1. Scale bar, 10 μm. (D) Immunostaining of GFP and α-tubulin in HeLa-K cells expressing GFP, GFP-hCMTBD, and GFP-N1 after cold treatment on ice for 1 h. Scale bar, 10 μm.