Table 2. Nucleotide diversity test of neutrality and rate of recombination test of vaccine candidate genes in Indian P. falciparum isolates.

Gene	No. of Allelic types	S	θ	π	Transitions	Transversions	dN	dS	dN/dS ratio	Tajima's D	PHI test P value
pfcsp	22	55	0.02473	0.02143	6.329	6.7402	0.0087	0.0788	0.110	-0.529924	0.000
pfglurp	59	21	0.00854	0.00524	0.6166	2.15546	0.0038	0.0072	0.528	-1.209877	0.3584
pfmsp1- K1	21	24	0.01728	0.00899	1.3857	2.0857	0.0103	0.0055	1.873	-1.830012	0.0380
pfmsp1- MAD20	26	38	0.02853	0.03030	5.329	5.246	0.0330	0.0295	1.119	0.233564	0.000
pfmsp1- RO33	8	12	0.01169	0.01380	3.607	1.857	0.0137	0.0149	0.919	0.909062	0.0150
pfmsp2-3D7/IC1	38	53	0.02502	0.033894	9.3058	7.776	0.0415	0.0039	10.641	1.275263	0.000
pfmsp2-FC27	12	59	0.03527	0.03457	7.015	12.136	0.0376	0.0318	1.182	-0.091029	0.0248
pfs25	3	2	0.00287	0.00287	0.6666	0.6666	0.0036	0.0000	∞	n/c

S: Segregating sites; θ: Hetrozygosity per site; π: Average Nucleotide diversity; dN: Average number of non synonymous mutation; dS: Average number of synonymous mutation