| Antibodies |
| Rabbit polyclonal anti-Kibra |
this paper |
N/A |
| Alexa Fluor 647-conjugated goat anti-rabbit antibody |
ThermoFisher |
A-21245; RRID: AB_2535813 |
| Chemicals, Peptides, and Recombinant Proteins |
| Potassium chloride (KCl) |
Sigma |
P5405-500G; CAS: 7447-40-7 |
| Potassium acetate (K-acetate) |
Sigma |
P1190-500G; CAS: 127-08-2 |
| Potassium HEPES (K-HEPES) |
Sigma |
H0527-100G; CAS: 82207-62-3 |
| Serotonin creatinine sulfate monohydrate (5-HT) |
Sigma |
H7752-250MG; CAS: 61-47-2 |
| Fast Green FCF |
Sigma |
F7252-5G; CAS: 2353-45-9 |
| L-15 Medium (Leibovitz) |
Sigma |
L5520-500ML |
| Rapamycin |
Calbiochem |
Cat# 553211 |
| Hemolymph |
From adult Aplysia californica
|
N/A |
| Artificial sea water from Instant Ocean sea salt |
Instant Ocean |
Product No. SS15-10 |
| Critical Commercial Assays |
| MidiPrep for generating DNA for injections |
Invitrogen |
K210004 |
| Experimental Models: Organisms/Strains |
| Aplysia californica |
NIH/University of Miami national Resource for Aplysia
|
N/A |
| Oligonucleotides |
| KIbra start inside for cloning GGGTCTAGAGAAATATGCCAGAGAGGGGCAG |
this paper |
N/A |
| KIbra start outside for cloning GTTTAGATGAAATATGCCAGA |
this paper |
N/A |
| KIbra end outside for cloning CAACAGAAACTCCACCAAGCT |
this paper |
N/A |
| Kibra end inside for cloning GGGGGATCCGCCTGCTTACACTTCTTCACC |
this paper |
N/A |
| Kibra outside reverse for mutation CACTCAGCCAACTTGGCAACT |
this paper |
N/A |
| Kibra reverse for mutation AGCTAGAGCTCGAGCGCTGACAGTATTTCTCTGA |
this paper |
N/A |
| KIbra forward for mutation |
this paper |
N/A |
| GCTCGAGCTCTAGCTTGGAAGCGGGCTGATGGCA |
this paper |
N/A |
| KIbra outside forward for mutation ACACGCTGAGGGAAGTGAAG |
this paper |
N/A |
| Recombinant DNA |
| pNEX mRFP-dn PKM Apl I |
[21] |
N/A |
| pNEX mRFP-dn PKM Apl III |
[20] |
N/A |
| pNEX mRFP-PKM Apl I |
[21] |
N/A |
| pNEX mRFP PKM Apl II |
[22] |
N/A |
| pNEX mRFP PKM Apl III |
[20] |
N/A |
| pNEX Kibra |
this paper |
N/A |
| pNEX dn Kibra |
this paper |
N/A |
| pNEX eGFP |
[49] |
N/A |
| pNEX mRFP |
[50] |
N/A |
| pNEX dn ApCCAL1 calpain |
[21] |
N/A |
| pNEX dn ApSOL calpain |
[21] |
N/A |