Diaphragm (DIA) cross-sections showing IgG staining (white arrow) in C57BL/10 (A), saline-treated mdx mice (B), prednisone-treated mdx mice (C) and diacerhein-treated mdx mice (D). The graphs (E) show the IgG staining (%) in the DIA muscle of C57BL/10 mice (Ctrl), saline-treated mdx mice (mdx), prednisone-treated mdx mice (mdxP) and diacerhein-treated mdx mice (mdxD). The graphs (F) show the creatine kinase (CK) levels in Ctrl, mdx, mdxP and mdxD. CK values expressed as mean ± standard deviation (SD). DIA cross-sections showing fibers which central nuclei (black arrow head) and peripheral nuclei (black arrow) in Ctrl (G), mdx (H), mdxP (I) and mdxD (J). The graphs show the nuclei central fibers (K) and peripheral nuclei fibers (L) in the DIA muscle of Ctrl, mdx, mdxP and mdxD groups. aP ≤ 0.05 compared with Ctrl mice, bP ≤ 0.05 compared with mdx mice (one-way ANOVA with Tukey’s post-hoc test).