Figure 3.

Processing and degradation of hTR. Screen shots of the hTR gene and RNAseq data from.⁷ (A) hTR maturation depends on the PAXT-independent poly(A) tail recognition by PABPN1 and the ensuing 3′end trimming by PARN (see text for details). Accordingly, depletion of PABPN1 (‘PABPN1 Kd’), but not ZFC3H1 (‘ZFC3H1 k_d’), decreases levels of mature hTR. Orange dashed lines indicate the maximum RNA signal levels in PABPN1 depletion conditions. (B) Extended hTR 3′ends (highlighted by red box) of impaired hTR precursors are targets for NEXT-dependent exosome degradation in the nucleoplasm.