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After osteogenic induction periods of 5 and 10 days, osteogenic-related gene expression was quantified by real-time quantitative polymerase chain reaction. COL, RUNX2, and ALP are shown in A–C, respectively. *P<0.05, N=5.
Abbreviations: ALP, alkaline phosphatase; COL, collagen; DLMS, direct laser metal sintering; MAO, microarc oxidation; RUNX2, runt-related transcription factor 2.
