Figure 1.

Batch incubation of human islets treated with PIC with or without 12-LO inhibitor. (a) GSIS was compared between human islets that were untreated (Cont), pretreated with PIC, or pretreated with PIC plus ML355 for 24 hours. GSIS was performed in the absence of PIC or ML355. Insulin secretion was expressed by taking the average insulin secretion of Cont at 1 mmol/L glucose as 1. (b) The insulin stimulation index was defined as described in the Methods. (c) GSIS was compared between untreated human islets (Cont) and islets treated with ML355 for 24 hours in the absence of PIC. Data are expressed as described in (a). (d) Insulin content in islet pellets was collected after GSIS. (e) Ratio of insulin content in the islet pellets. Each condition from a single donor was performed in triplicate, and data from three to four donors were combined (n = 8 to 12). *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.001 by Student t tests. a: P < 0.05 one-way analysis of variance with Dunnett’s posttest in comparison with Cont 1, mmol/L glucose. b: P < 0.05 one-way analysis of variance with Dunnett’s posttest in comparison with Cont, 18 mmol/L glucose. ns, not statistically different.