Figure 2.

Perifusion of human islets treated with PICs with or without 12-LO inhibitor. (a) A representative figure (donor 3) comparing perifusion of human islets that were untreated (Cont), treated with PIC, or treated with PIC in the presence of ML355 for 24 hours before perifusion. Data were expressed by taking insulin secretion at 3 mmol/L glucose (base) as 1. (b–g) Stimulation index, the first-phase response, and the second-phase response (as defined in the Methods) were obtained for six donors, as shown in (b), (d), and (f). Each parameter was also compared by taking the response of PIC as 1 in (c), (e), and (g) (n = 6). a: P < 0.05 vs PIC by Friedman test with Dunn’s multiple comparison test as posttest.