Figure 6. Expression of profibrotic genes in lung tissues of mice with pulmonary fibrosis.
Pulmonary fibrosis was induced by intratracheal instillation of 1.5 U/kg of bleomycin. Mice were treated with substances indicated twice a week for three weeks starting one day later after the bleomycin administration. At the end of treatment, lungs were harvested and homogenized The expression of genes encoding transforming growth factor-β cytokines (TGFB1-3), transforming growth factor-β receptors (TGFBR1-2) and smooth muscle α actin (CTGF) was measured by the QPCR and presented as fold change relative to the healthy animals. Means ± SD are shown. *P < 0.05 when compared with control mice.