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. Author manuscript; available in PMC: 2017 Aug 7.
Published in final edited form as: J Neurosci Methods. 2014 Nov 8;240:22–27. doi: 10.1016/j.jneumeth.2014.10.015

Table 1.

Injection rates at 10, 7.5, 5.0, 2.5 and 1.0 μl/min at Injection Volumes 100, 50, 25 and 10 μl. Ipsilateral and Contralateral staining of the Middle Cerebral Artery (MCA) at specific injection rates and volumes, no staining at the 1.0 μl/min injection rate(*) due to opposing pressure from the CCA. No further testing of injection rate 1.0 μl/min due to inability to overcome opposing pressure. Little to no contralateral staining was seen at the injection rate 2.5 μl/min at volumes 10 and 25 μl. Slight staining (**) was seen in the frontal region of both hemispheres due to mice having an azygous Anterior Cerebral Artery. Carbon black ink was found throughout the liver and vasculature at injection rates 10, 7.5 and 5.0 μl/min and injection volumes 100 and 50 μl with slight staining at 25 μl and no staining at 10 μl.

Injection rate (μl/min) Injection volume (μl) MCA staining
Hemispheric staining
Systemic staining
Ipsilateral Contralateral Ipsilateral Contralateral Liver
10.0 100 Yes Yes Yes Yes Yes
  7.5 100 Yes Yes Yes Yes Yes
  5.0 100 Yes Yes Yes Yes Yes
  2.5 100 Yes Yes Yes Yes Yes
  1.0* 100 N/A N/A N/A N/A N/A
10.0   50 Yes Yes Yes Yes Yes
  7.5   50 Yes Yes Yes Yes Yes
  5.0   50 Yes Yes Yes Yes Yes
  2.5   50 Yes Yes Yes Yes Yes
  2.5   25 Yes Slight Yes Slight Slight
  2.5   10 Yes No Yes Slight** No