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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: J Immunol Methods. 2017 May 26;448:66–73. doi: 10.1016/j.jim.2017.05.010

Figure 3. Isolation of PfTRAP antigen-positive B cells.

Figure 3

Splenocytes were harvested from PfTRAP-immunized mice, subjected to B cell enrichment, and stained for FACS analysis. Gates were set as follows: A) total lymphocytes, B) cell singlets, C) B220+ B cells (Pacific Blue), D) CD38+ (APC), E) IgM– and IgD– (FITC and AF700, respectively), and F) PfTRAP+ (BV785) and irrelevant protein decoy– (BV510).