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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: J Immunol Methods. 2017 May 26;448:66–73. doi: 10.1016/j.jim.2017.05.010

Figure 5. BLI analysis of recombinant anti-PfTRAP mAbs.

Figure 5

AMC biosensors derivatized with recombinant mAbs were incubated for 300 s in well solutions containing serially diluted PfTRAP to produce color-coded sensorgrams (colors correspond to the PfTRAP concentrations, see legend). The sensors were then transferred into solutions without PfTRAP and the complexes were allowed to dissociate for another 300 s. Each of the datasets was globally fitted to produce the red trendlines and the estimated on- and off-rates from which KD estimates were calculated. A) AKBR1, B) AKBR2, C) AKBR3, D) AKBR5, E) AKBR4, F) AKBR6.