Skip to main content
. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Methods. 2017 Apr 13;125:70–83. doi: 10.1016/j.ymeth.2017.04.005

Fig. 6. A scheme of the isolation of native spliceosomes.

Fig. 6

Treatment of supraspliceosomes with EDTA induces a conformational change (please note that this step is reversible) [28]. This change makes the pre-mRNA available for base pairing with an oligodeoxynucleotide complementary to abundant intronic sequences that conform to the 5’SS consensus sequence. This part of the scheme is oversimplified because some components dissociate upon EDTA treatment but reassociate upon addition of magnesium cations. Subsequent treatment with RNase H and readdition of Mg2+ yield native spliceosomes (please note that this step is irreversible). Adapted from Ref. [37].