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. 2001 May 15;29(10):e49. doi: 10.1093/nar/29.10.e49

Figure 1.

Figure 1

Representative negative ion MALDI mass spectra of (A) tRNAVal1 (theoretical Mr 24 643 Da, experimental Mr 24 705 Da) and (B) tRNAVal1 after CMC derivatization (theoretical Mr 24 895 Da, experimental Mr 24 950 Da). The tRNA samples were analyzed using 2:1 25 mg/ml (v:v) THAP:diammonium hydrogen citrate with 1 mM imidazole (22). The mass shift between the two analytes is 250 ± 6 u as determined from replicate measurements. The expected mass shift due to the presence of one CMC-derivatized pseudouridine residue is 252 u.