Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Aug 7;7:7454. doi: 10.1038/s41598-017-07614-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Detection of nascent phospholipids by pulse labelling with deuterated choline during NR formation. (a) Representative backscattered electron (BSE) image of a saquinavir-treated mouse preadipocyte (black arrowheads point towards high membrane curvature indicating NR initiation sites) with a corresponding image from NanoSIMS showing enrichment of deuterium signal (²H/¹H ratio) revealing nascent phospholipids incorporated directly into forming NR (white arrowheads). (b) BSE and NanoSIMS images of a control cell treated with DMSO vehicle showing lack of NR tubules and no deuterium enrichment within the nucleus, and undistorted nuclear boundary. Colour scale of NanoSIMS images 2–20 equals 0.02–0.2% of ²H/¹H ratio.