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. 2017 Aug 7;7:7454. doi: 10.1038/s41598-017-07614-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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3D NanoSIMS of nascent phospholipid distribution during NR formation. (a) Representative backscattered electron (BSE) image of a saquinavir-treated mouse preadipocyte pulsed labelled with deuterated choline; scale bar 2 µm. (b) 3D reconstruction of NanoSIMS analysis of subsequent layers of the specimen shown in (a). (c) Panel of NanoSIMS images used for the 3D reconstruction shown in (b); examples of tip and base region of NR tubules are indicated by arrowheads and arrows, correspondingly; a single image corresponds to ~10 nm specimen thickness. Colour scale of NanoSIMS images 2–20 equals 0.02–0.2% of ²H/¹H ratio. See corresponding Supplementary Movies 2 and 3.