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. 2017 Aug 7;7:7454. doi: 10.1038/s41598-017-07614-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of the nascent phospholipid enrichment at NR structures in the NanoSIMS experiments. (a) Scatter plot showing maximum intensity of ²H/¹H ratio at the bulk nuclear envelope (NE), and the tip and base regions of NR invaginations in mouse preadipocytes pulse labelled with deuterated choline chloride; mean ± SD indicated; ****p < 0.0001. (b) Examples of ratiometric images used for quantification of the ²H/¹H ratios with indicated line profiles corresponding to the base region (outer arrows) or tip (middle arrow) of NR tubules; N, nucleoplasm; C, cytoplasm. Note the different appearance of these images from NanoSIMS images presented in other figures, due to using ratiometric images for measurements rather than HSI images. (c) Plot profile of ²H/¹H ratio along a typical invagination shown in (d); asterisks correspond to focal PC inclusion sites along the tubule length. Colour scale of 0–30 equals 0–0.3% of ²H/¹H ratio.