Figure 3.

Low-toxicity isoform-targeted compounds induce HIV-1 gag. (a–c) EC₅₀ measurements for largazoles. JLAT10.6 cells were incubated with indicated compounds for 24 h at increasing concentrations and FACS performed to quantify the percent of GFP positive cells. (d) Largazole induces HIV Gag proteins. JLAT10.6 cells were incubated with SDL148 for 24 h and Western blot performed for HIV Gag proteins. (e) Toxicity profile of largazoles. JLAT10.6 cells were incubated with compounds at indicated concentrations and MTT toxicity assay performed as described in Methods. Data indicates mean values, and error bars indicate mean ± s.e.m. (n ≥ 3).