Figure 5.

Largazoles are isoform-targeted class I HDAC inhibitors that enhance histone H3 acetylation at the HIV promoter. Largazoles inhibit class I, but not class II histone deacetylases. Compounds at indicated concentrations were incubated with (a) Jurkat or (b) primary T-cells for 8 h and Western blot performed for acetylated histone H3 (a marker of class I HDAC inhibition) or acetylated tubulin (a marker for HDAC6, a class II HDAC) inhibition. (c) Chromatin immunoprecipitation (ChIP) of acetylated histone H3 and HIV LTR promoter. JLAT10.6 cells were incubated with DMSO or 1 μM of SDL148 for 6 h and ChIP performed as described in Methods. Data indicate mean values and error bars indicate mean ± s.e.m. (n ≥ 3). *p < 0.05, (Student’s t-test). See also Supplementary Fig. S1.