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. 2017 Aug 7;7:7471. doi: 10.1038/s41598-017-08264-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Decreased numbers of alveolar macrophages in Abt mice. C57BL/6 mice in the Abt group were given 1 g/L ampicillin, 0.5 g/L vancomycin, 1 g/L neomycin sulfate and 1 g/L metronidazole in their drinking water for five weeks. (a) Detection of alveolar macrophages (F4/80^hi CD11c^hi) in mouse lung MNCs by FACS. (b) Frequency (alveolar macrophages/lung mononuclear cells) and number of alveolar macrophages (F4/80^hi CD11c^hi) were analyzed. The MNCs were gated by FSC and SSC. (c) Immunofluorescence analysis of alveolar macrophages (F4/80^hi CD11c^hi), indicated by arrows (magnification × 63). (d) F4/80^hi CD11c^hi cell numbers were counted and analyzed in 50 fields (63×) per tissue slide. There were five mice in each group. Data are shown as mean ± SEM. *p < 0.05 compared to the control group.