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. 2017 Aug 7;7:7471. doi: 10.1038/s41598-017-08264-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Altered gene expression in alveolar macrophages isolated from Abt mice. The mice in Abt group were given antibiotics for five weeks. Purified alveolar macrophages (F4/80^hi CD11c^hi) were analyzed by GeneChip. (a) Alveolar macrophages (F4/80^hi CD11c^hi) were sorted from lung MNCs. (b) The heatmap showed the differential expression of the indicated genes between the Abt group and the control group. DEGs were determined by Limma, and were row-based median normalized (2 samples/group, 15 mice/sample). (c) Protein-protein interaction (PPI) networks of the DEGs identified in the Abt group compared with the control. The red nodes indicate the up-regulated DEGs and the blue nodes indicate the down-regulated DEGs. Proteins associated with each other are linked by an edge. The degree of color represents the DEG fold change in reference to the scaleplate. (d) The mRNA expression levels of selected DEGs in the sorted macrophage cells (F4/80^hi CD11c^hi) were measured by real-time PCR (n = 3). *p < 0.05 compared with the control group.