Table 1.
Primers used for RT-PCR.
| GS | Type | Primers (5′–3′) | RV | GBA |
|---|---|---|---|---|
| PB1 | F | aaagcaggcaaaccatttg | A/swine/Korea/S452/2004(H9N2) | AY790312.1 |
| R | ggcattttttcatgaaggaca | |||
| PB2 | F | aaagcaggtcaattatattc | A/chicken/Shandong/B1/1998(H9N2) | EU914196.1 |
| R | agtagaaacaaggtcgtttttaaac | |||
| PA | F | aaagcaggtactgatcc | A/chicken/Guangdong/TS/2004(H9N2) | JQ639777.1 |
| R | agtagaaacaaggtacttttttg | |||
| HA | F | agcaaaagcaggggaatttcac | A/chicken/Shaanxi/xy1/2012(H9N2) | KF638575.1 |
| R | agtagaaacaagggtgtttttgcc | |||
| NP | F | atggcgtctcaaggcaccaaa | A/swine/Korea/S452/2004(H9N2) | AY790308.1 |
| R | tctttaattgtcatactcctctgca | |||
| NA | F | agcaaaagcaggagtaaaaatg | A/chicken/Shandong/B4/2007(H9N2) | EU346938.1 |
| R | caaggagtttttttttaaaattgc | |||
| M | F | gcaggtagatatttaaagatgagtc | A/swine/Korea/S452/2004(H9N2) | AY790306.1 |
| R | acaaggtagttttttactccagttc | |||
| NS | F | agcgaaagcagggtgacaa | A/swine/Korea/S452/2004(H9N2) | AY790309.1 |
| R | tagaaacaagggtgttttttatca |
GS, Gene segment; RV, Reference viruses; GBA, GenBank accession no.