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. 2017 Aug 7;7:7467. doi: 10.1038/s41598-017-07940-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ribeye is dynamic and mobile within the ribbon. (a) Ribbon schematic showing measurement regions: the whole ribbon (dashed red), within the bleached (dashed black) and unbleached (dashed gray) hemispheres. (b) Normalized fluorescence profiles for each region in a following bleaching of half the ribbon (at 0 s) for an example ribbon (same as in d). (c) Average normalized recovery curve for bleached hemisphere (n = 21 ribbons; red: exponential fit). (d) Pixel intensity profiles (left) from regions between brackets in images (right) before (i) and after (ii) bleaching. The bottom profile and image (iii) show the average change in pixel intensity for each pixel over successive images during the first 50 time points (~17 s) of recovery. Gray indicates no average change, while brighter and darker values indicate gain and loss of fluorescence, respectively. (e) Pixel intensity profiles (mean ± SD, n = 8 ribbons) across the center of ribbons before (black), immediately after (red), and 250 s after (blue) bleaching. Ribbons diameters were normalized to the same width; the peaks of pixel intensities were normalized. (f) Average recovery of the bleached hemisphere (from c) compared to particle simulations with different diffusion coefficients (colored traces). Each simulation trace is an average of 20 runs. See also Supplementary Fig. S2, Supplementary Movie S1.