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Genome organization in the ftac88 vector. Most of the tetR gene was exchanged for a linker containing the AvaII site, thus eliminating the second SnaBI site previously situated in this region (for comparison see Fig. 1C). The modified pVIIISTS gene is situated between the duplicated overlapping promoter/terminator structures, without disrupting the two phage transcriptional units. Note the pVIIISTS gene is under the control of a tac promoter (Ptac).
