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. 2001 May 15;29(10):e50. doi: 10.1093/nar/29.10.e50

Figure 5.

Figure 5

(A) Binding of mAb GV4H3 to phages expressing the GV4H3 epitope. Equal amounts of phages were applied in duplicate to a nitrocellulose membrane filter and reacted with the GV4H3 mAb. The GV4H3 epitope displaying phages, ftac88(4H3) and fth1(4H3), showed strong signals. Phages ftac88 and fth1 were used as negative controls. (B) Deletion of the recombinant pVIII gene in ftac88(4H3). SnaBI/BamHI digests of ftac88 derived from DH5α (lane 1) or DH5αF′ (lane 2) were run on 0.8% agarose gel. SnaBI/BamHI digests of ftac88(4H3) isolated from DH5α (lane 3) or DH5αF′ (lane 4) are also shown. M, DNA ladder mix. The upper arrow indicates the tet insert deleted product and the lower arrow indicates the recombinant pVIII gene deleted fragment.