Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Aug 7;7:7450. doi: 10.1038/s41598-017-07077-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

The extracellular CRAC domain from SIDT1 is part of a vesiculation signal required for translocation to the plasma membrane. (A) Representative confocal images of HEK293 cells expressing the SIDT1 double mutant from the CRAC domains (dmSIDT1). The right panel shows the line analysis illustrating the lack of translocation of the dmSIDT1 upon MβCD treatment. The lower panel shows the mean ± standard deviation of the fluorescence intensity in the line before and after MβCD treatment obtained from at least 40 independent experiments. (B) Total internal reflection fluorescence microscopy (TIRFM) representative images obtained with HEK293 cells expressing the dmSIDT1. The right panel shows the mean ± standard deviation from the total fluorescence of at least 30 independent measurements. (C) Representative confocal images of HEK293 cells expressing the SIDT1 transmembrane mutant (tmSIDT1). Notice the normal translocation to the plasma membrane after MβCD treatment. (D) Total internal reflection fluorescence microscopy (TIRFM) representative images obtained with the trSIDT1 mutant. (E) Representative confocal images of HEK293 cells expressing the exSIDT1 mutant. Notice that this mutant does not translocate to the plasma membrane after MβCD treatment. (F) Total internal reflection fluorescence microscopy (TIRFM) representative images obtained with the exSIDT1 mutant. Ti, the initial time of recording and Tf, final recording time. Total fluorescence normalized to Ti. In all panels scale bar: 10 microns.